Presentations

    High Throughput Poration of Mammalian Cells using Femtosecond Laser-activated Plasmonic Substrates, at Tokyo Metropolitan University (Tokyo, Japan), Thursday, January 29, 2015:
    We present a new cell transfection method that uses femtosecond laser-excited localized surface plasmons (LSPs) on a nanostructured micropyramid array. Our gold-layered micropyramids have nano-apertures at the apex to form high local electric field enhancements, or “hot spots.” These hot spots form microbubbles that temporarily perforate mammalian cell (HeLa S3) membranes and allow dye molecules and plasmid vectors to diffuse through the membrane openings. We optimize our laser parameters for successful poration and high cell viability. Our nontoxic, efficient, and scalable technique... Read more about High Throughput Poration of Mammalian Cells using Femtosecond Laser-activated Plasmonic Substrates
    Plasmonic Cell Transfection using Micropyramid Arrays, at SPIE Photonics West, SPIE (San Francisco, CA), Sunday, February 8, 2015:
    We present a new cell transfection method that uses femtosecond laser-excited localized surface plasmons (LSPs) on a nanostructured micropyramid array. Our gold-layered micropyramids have nano-apertures at the apex to form high local electric field enhancements, or “hot spots.” These hot spots form microbubbles that temporarily perforate mammalian cell (HeLa S3) membranes and allow dye molecules and plasmid vectors to diffuse through the membrane openings. We introduce an emerald green fluorescent protein (EmGFP) reporter plasmid into the cells to determine the LSP-mediated transfection... Read more about Plasmonic Cell Transfection using Micropyramid Arrays