@article {Shen:166,
	title = {Ablation of cytoskeletal filaments and mitochondria in cells using a femtosecond laser nanoscissor},
	journal = {Mechanics and Chemistry of Biosystems},
	volume = {2},
	year = {2005},
	pages = {17{\textendash}26},
	abstract = {Analysis of cell regulation requires methods for perturbing molecular processes within living cells with spatial discrimination on the nanometer- scale. We present a technique for ablating molecular structures in living cells using low-repetition rate, low-energy femtosecond laser pulses. By tightly focusing these pulses beneath the cell membrane, we ablate cellular material inside the cell through nonlinear processes. We selectively removed sub- micrometer regions of the cytoskeleton and individual mitochondria without altering neighboring structures or compromising cell viability. This nanoscissor technique enables non-invasive manipulation of the structural machinery of living cells with several-hundred-nanometer resolution. Using this approach, we unequivocally demonstrate that mitochondria are structurally independent functional units, and do not form a continuous network as suggested by some past studies. Keywords: Nanoscissor; nanosurgery; femtosecond laser; photodisruption; cytoskeleton; mitochondria},
	url = {/files/mazur/files/rep_166.pdf},
	author = {Shen, N. and D. Datta and Schaffer, C. B. and LeDuc, P. and Ingber, D. E. and Mazur, E.}
}
