Femtosecond laser interactions with materials: from micromachining to microsurgery

When femtosecond laser pulses are focused tightly into a transparent material, the intensity in the focal volume can become high enough to cause nonlinear absorption of laser energy. The absorption, in turn, can lead to permanent structural or chemical changes. Such changes can be used for micromachining bulk transparent materials. Applications include data storage and the writing of waveguides and waveguide splitters in bulk glass, and fabrication of micromechanical devices in polymers.

We have used this technique to manipulate sub-cellular structures inside live and fixed cells. Using only a few nanojoules of laser pulse energy, we are able to selectively disrupt individual mitochondria in live bovine capillary epithelial cells, and cleave single actin fibers in the cell cytoskeleton network of fixed human fibro-blast cells. We have also used the technique to micromanipulate the neural network of C. Elegans, a small nematode. Our laser scalpel can snip individual axons without causing any damage to surrounding tissue, allowing us to study the function of individual neurons with a precision that was not achievable before.