Nanosurgery with femtosecond lasers
We have developed a technique to disrupt submicrometer-sized organelles within living cells or tissue without affecting the surrounding material or compromising viability of the cell or organism. When a femtosecond laser pulse is tightly focused into a nearly-transparent biological material, energy is deposited by nonlinear absorption only in the focus where laser intensity is high, resulting in disruption of the structure in the focal volume. Because the absorption is confined to the small focal volume, the surrounding material is unaffected, allowing micrometer precision in the disruption of biological tissue.
Subcellular surgery and nanosurgery,
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Chemistry Seminar, Middle Tennessee University,
Friday, February 12, 2021
Nanosurgery in live cells using ultrashort laser pulses,
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2005 SPIE Photonics West Conference (San Jose, CA),
Tuesday, January 25, 2005:
Probing cell mechanics with femtosecond laser pulses,
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Photonics West 2007 (San Jose, CA),
Sunday, January 21, 2007:
Manipulating cells using ultrashort laser pulses,
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Wednesday Night Research Seminar, Harvard University (Cambridge, MA),
Wednesday, September 9, 2009:
Subcellular surgery and nanosurgery,
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Western Washington University (Bellingham, WA),
Tuesday, May 13, 2014:
Photodisruption in biological samples using femtosecond laser pulses,
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Photonic West Conference (San Jose, CA),
Tuesday, January 23, 2001:
Subcellular surgery and nanosurgery,
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CIMIT/Lester Wolfe Workshop on Femtosecond Microscopy & Microsurgery, Wellman Center for Photomedicine (Boston, MA),
Tuesday, April 18, 2006:
Subcellular surgery and nanosurgery,
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Phi Beta Kappa Lecture, Pomona College (Claremont, CA),
Tuesday, April 15, 2008:
Subcellular surgery and nanosurgery,
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Special Laser Seminar / NCCR MUST Seminar, ETH Zürich (Zürich, Switzerland),
Thursday, February 23, 2012:
High Throughput Poration of Mammalian Cells using Femtosecond Laser-activated Plasmonic Substrates,
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Tokyo Metropolitan University (Tokyo, Japan),
Thursday, January 29, 2015:
Femtosecond laser dissection of neurons in C. elegans,
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Industrial Outreach Program, Harvard University (Cambridge, MA),
Wednesday, April 27, 2005:
Subcellular surgery and nanosurgery,
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Zhejiang University (Hangzhou, China),
Thursday, March 29, 2007:
Subcellular surgery and nanosurgery,
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PR-LSAMP Role Model Series, UPR Rio Piedras (Rio Piedras, PR),
Friday, February 19, 2010:
Shining light on cells to cure diseases,
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SPIE LASE Photonics West 2017 (San Francisco, CA),
Sunday, January 29, 2017: